Oxylipins signature to monitor the cardiometabolic status and its response to dietary intervention
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1. Overall project description
Rational & Objectives. There is an urgent need to find reliable early biomarkers of the cardiometabolic syndrome allowing intervention before irreversible damage develops while assessing the efficacy of nutritional prevention. Oxylipins could be relevant candidate biomarkers thanks to their involvement in the homeostatic regulation of various process related to the cardiometabolic status and the consistent link reported between diet, oxylipins and cardiometabolic dysregulations.
Our main objective in the OXYGENATE project is to uncover and validate the oxylipin signatures reflecting the cardiometabolic status or its early transition towards the cardiometabolic syndrome. To achieve this goal, we proposed a multidisciplinary project involving experts in lipidomics, nutrition and epidemiology and combining the comprehensive and quantitative lipidomic profiling of circulating oxylipins and the use of different biobanks from large prospective cohorts and different whole-diet interventions.
Overall achievements. The OXYGENATE project started with the optimization of the MS-base targeted lipidomic profiling of oxylipins. The optimized method developed allows the analysis of over 160 different oxylipins representative of all families of oxylipins. The method has been validated through a round robin test involving 5 different laboratories worldwide and allowing to determine for each oxylipin (i) intra- and inter-day variabilities and (ii) consensus values and their respective dispersion. The OXYGENATE consortium will also provide a detailed SOP for sample storage and preparation including quantitative and qualitative data regarding oxylipin stability. In order to identify candidate oxylipins differentiating individuals at different stages of cardiometabolic syndrome and individuals with different quality of dietary patterns, cross-sectional and longitudinal case-control studies have been performed selecting participants from the polish branch of the PURE cohort. Validation consists in replicating the cross-sectional studies with the participants of the French NutriNet-Santé cohort. The reliability of the candidate oxylipins identified will be further validated by investigating their relationships with the evolution of cardiometabolic syndrome in response to diet using the biobanks from the Danish SHOPUS and the American iMAPS dietary intervention studies.
Expected impacts. On completion, the OXYGENATE project will have (i) provided an internationally validated method of comprehensive oxylipins profiling (ii) generated the 1st large diet- and health-related oxylipins database, (iii) identified and validated the oxylipins reflecting the quality of dietary patterns and the cardiometabolic status and (iv) provided a proof-of-concept of the ability of oxylipins to monitor the effect of diet on the cardiometabolic status. It is expected that the oxylipins identified and validated will allow a better detection and management of the cardiometabolic syndrome.
THe OXYGENATE project will provided the first internationally validated method of comprehensive oxylipins profiling
4.1 List of publications
|Authors||Title||Year, Issue, PP||Doi|
|Cécile Gladine, Annika I. Ostermann, John W. Newman, Nils Helge Schebb||MS-based targeted metabolomics of eicosanoids and other oxylipins: Analytical and inter-individual variabilities||https://doi.org/10.1016/j.freeradbiomed.2019.05.012|
4.2 Presentation of the project
|Target group||Authors||Means of communication||Hyperlink|
|Scientists, PhD students and students||E. Koch, A. I. Ostermann, M. Mainka, C. Dalle, T. Konrad, C. Gladine, N. H. Schebb, Quantification of Total Oxylipins in Plasma – Challenges & Strategies for Hydrolysis and Solid Phase Extraction. 47th International Conference on Food Chemistry and Technology, Berlin, Germany, 2018.||Poster|
|Scientists, PhD students and students||E Koch, AI. Ostermann, M Mainka, C Dalle, T Konrad, C Gladine, NH Schebb, Extraction of lipids and oxylipins from plasma for quantification of total oxylipins – Challenges and strategies, 7EWLM, Brussels, 2018||Poster|
|Scientists, PhD students and students||E. Koch, A. I. Ostermann, M. Mainka, C. Dalle, C. Gladine, N. H. Schebb, Challenges and strategies for the quantification of total oxylipins in biological samples, Regional conference of food chemistry, Wuppertal, Germany, 2019||Poster|
|Scientists, PhD students and students||C. Dalle, M. Mainka, J. Dalloux-Chioccioli, A.I. Ostermann, M. Rothe, J.W. Newman, J. Bertrand-Michel, C. Gladine, N.H. Schebb. Comparaison inter-laboratoires de profils d’oxylipines par lipidomique ciblée dans le cadre du projet JPI-OXYGENATE, RFMF, France, 2019||Poster|
4.3 List of submitted patents and other outputs
|Patent licence||Partners involved||Year||International eu or national patent||Comment|