Di-Mi-Liv

Genetic predisposition and general overnutrition are discussed as key factors in the development of non-alcoholic fatty liver disease (NAFLD); however, alterations of intestinal microbiota composition and associated impairments of intestinal barrier function are thought to be critical in the onset and progression of NAFLD. Also studies suggest a pivotal role of bile acids and microbial bile acid metabolism as mediators of gut-liver-crosstalk, subsequently affecting NAFLD initiation and progression. Soluble fibers like oat β-glucans bind bile acids and have been proposed to modulate intestinal microbiota composition, thereby affecting metabolic parameters and liver health. Based on this background, the main aim of the transnational Di-Mi-Liv project was to reveal a causal relationship between specific diet-responsive microbial taxa and alterations of the bile acid pool as risk factor for initiation and progression of metabolic liver diseases. Herein, the effects of the soluble fiber oat β-glucan were assessed. Key findings achieved within the project include that in patients with NAFLD fiber intake is ~12g/d lower than that recommended by the nutrition societies. Also, with the help of the two high-fiber flake mixes employed in the intervention study, the daily fiber intake could be significantly increased almost to the level of the recommendations during the intervention phase without significantly changing the general dietary pattern. In the groups consuming insoluble fibers, this was associated with a marked decrease of transaminase activity and concentration of TLR ligands in blood while fecal microbiota and bile acid composition was widely unaffected. In patients, the intake of oat β-glucan rich flakes had limited effects on liver parameters and fecal microbiota composition. In contrast, in mouse studies employing models of early and late stages of NAFLD a diet enriched with oat β-glucan (~30% of total fiber intake) markedly protected mice from the development of early stages of NAFLD. The protection from liver disease progression and liver fibrosis seemed to be dependent on the action of oat β-glucan on intestinal microbiota and intestinal FXR-activation.

In summary, the results of our project suggest that oat β-glucan may have some protective effects on the development of metabolic diseases. However, in settings of a pre-existing NAFLD, it seems that an increase in the intake of other fibers like insoluble spelt bran might be more beneficial.